Authors: Rubio, Natalie R.; McCartney, Naya E.; Trimmer, Barry A.; Kaplan, David L.
Keywords: cellular agriculture, cultured meat, Insect cell culture, Tissue engineering, Bioactuation, Vaccine production, Robotics, Advanced manufacturing
Insect cells may be preferred over mammalian cells for biofabrication because of several bioprocess benefits including tolerance to fluctuations in the external environment, low secretion of and sensitivity to toxic by-products and ease of genetic modification. Insect muscle cells, in particular, are functionally promising in vitro but have yet to find a purpose outside of basic research. Insect muscle cell development and physiology are well-documented and myogenic cell populations from a variety of species and tissue types have been propagated in vitro. Muscle cells can be easily isolated from insect embryos or metamorphosing stages, separated from contaminating cell types and triggered to differentiate via administration of insect-specific hormones. The abilities of insect cells to grow under ambient conditions, survive and function (i.e., contract) for extended periods of time without fresh nutrients and to exhibit powerful contractions present an attractive alternative to mammalian cell culture in the context of advanced manufacturing processes. Moreover, insect cells are less costly to produce at large-scale, lowering barriers to commercialization. Bioactuation devices, cultured meat and ingestible vaccines have been identified as promising areas of application for insect cell cultivation, with others likely to emerge. Some of the next steps to advance insect cell-based technologies include the design of control systems to regulate in vitro contractions, adaptation of tissue engineering techniques for invertebrate cells, scaling insect cell and tissue formation to meet the needs of these broader applications, and evaluation of food nutrition and safety.
