Authors: Thyden, R., Perreault, L. R., Jones, J. D., Notman, H., Varieur, B. M., Patmanidis, A. A., Dominko, T., & Gaudette, G. R.
Rapidly expanding skeletal muscle satellite cells with cost-effective methods have been presented as a solution for meeting the growing global demand for meat. A common strategy for scaling cell proliferation employs microcarriers, small beads designed to support anchorage-dependent cells in suspension-style bioreactors. No carrier has yet been marketed for the cultivation of lab-grown meat. The objective of this study was to demonstrate a rapid, food safe, decellularization procedure to yield cell-free extracellular matrix scaffolds and evaluate them as cell carriers for lab grown meat. Broccoli florets were soaked in SDS, Tween-20, and bleach for 48 h. The decellularization process was confirmed via histology, which showed an absence of cell nuclei, and DNA quantification (0.0037 ± 0.00961 μg DNA/mg tissue). Decellularized carriers were sorted by cross sectional area (7.07 ± 1.74 mm2, 3.03 ± 1.15 mm2, and 0.49 ± 0.3 mm2) measured for eccentricity (0.73 ± 0.16). Density measurements of decellularized carriers (1.01 ± 0.01 g/cm) were comparable to traditional microcarriers. Primary bovine satellite cells were inoculated into and cultured within a reactor containing decellularized carriers. Cell adhesion was observed and cell death was limited to 2.55 ± 1.09%. These studies suggested that broccoli florets may serve as adequate edible carrier scaffolds for satellite cells.
The full PDF is available at this link: https://doi.org/10.3390/app12105155
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* Cite as: Thyden, R., Perreault, L. R., Jones, J. D., Notman, H., Varieur, B. M., Patmanidis, A. A., Dominko, T., & Gaudette, G. R. (2022). An Edible, Decellularized Plant Derived Cell Carrier for Lab Grown Meat. Applied Sciences, 12(10). https://doi.org/10.3390/app12105155
* License: Creative Commons Attribution 4.0 International
